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twf2 rabbit polyclonal  (Novus Biologicals)
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Novus Biologicals twf2 rabbit polyclonal
Relative protein abundance of <t>TWF2</t> positively correlates with spine length. A , Module 16 eigenprotein abundance exhibits a significant positive correlation with dendritic spine length. B , Module 16 eigenprotein abundance is significantly positively correlated with thin spine length. Each point represents one case. N = 45 (17 Control, 6 CAD, 22 AD). C , Gene ontology (GO) for Module 16 indicates involvement in protein folding. Module 16 eigenprotein abundance does not differ among control, CAD, and AD synaptosomes. N = 57 (19 Control, 7 CAD, 31 AD). Error bars indicate the 25th and 75th percentiles. D , The top 6 hub proteins for Module 16 are shown. TWF2 is the top hub protein. E , Log 2 -transformed relative protein abundance of TWF2 positively correlates with spine length. F , Log 2 -transformed relative protein abundance of TWF2 was plotted against thin spine length. For E , F , points represent individual cases and the best fit line was determined via linear model. For A , B , E , F , correlations were assessed by biweight midcorrelation (bicor).
Twf2 Rabbit Polyclonal, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/twf2 rabbit polyclonal/product/Novus Biologicals
Average 92 stars, based on 1 article reviews
twf2 rabbit polyclonal - by Bioz Stars, 2026-02
92/100 stars
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twfz rabbit polyclonal  (Novus Biologicals)
92
Novus Biologicals twfz rabbit polyclonal
Relative protein abundance of <t>TWF2</t> positively correlates with spine length. A , Module 16 eigenprotein abundance exhibits a significant positive correlation with dendritic spine length. B , Module 16 eigenprotein abundance is significantly positively correlated with thin spine length. Each point represents one case. N = 45 (17 Control, 6 CAD, 22 AD). C , Gene ontology (GO) for Module 16 indicates involvement in protein folding. Module 16 eigenprotein abundance does not differ among control, CAD, and AD synaptosomes. N = 57 (19 Control, 7 CAD, 31 AD). Error bars indicate the 25th and 75th percentiles. D , The top 6 hub proteins for Module 16 are shown. TWF2 is the top hub protein. E , Log 2 -transformed relative protein abundance of TWF2 positively correlates with spine length. F , Log 2 -transformed relative protein abundance of TWF2 was plotted against thin spine length. For E , F , points represent individual cases and the best fit line was determined via linear model. For A , B , E , F , correlations were assessed by biweight midcorrelation (bicor).
Twfz Rabbit Polyclonal, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/twfz rabbit polyclonal/product/Novus Biologicals
Average 92 stars, based on 1 article reviews
twfz rabbit polyclonal - by Bioz Stars, 2026-02
92/100 stars
  Buy from Supplier

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Relative protein abundance of TWF2 positively correlates with spine length. A , Module 16 eigenprotein abundance exhibits a significant positive correlation with dendritic spine length. B , Module 16 eigenprotein abundance is significantly positively correlated with thin spine length. Each point represents one case. N = 45 (17 Control, 6 CAD, 22 AD). C , Gene ontology (GO) for Module 16 indicates involvement in protein folding. Module 16 eigenprotein abundance does not differ among control, CAD, and AD synaptosomes. N = 57 (19 Control, 7 CAD, 31 AD). Error bars indicate the 25th and 75th percentiles. D , The top 6 hub proteins for Module 16 are shown. TWF2 is the top hub protein. E , Log 2 -transformed relative protein abundance of TWF2 positively correlates with spine length. F , Log 2 -transformed relative protein abundance of TWF2 was plotted against thin spine length. For E , F , points represent individual cases and the best fit line was determined via linear model. For A , B , E , F , correlations were assessed by biweight midcorrelation (bicor).

Journal: The Journal of Neuroscience

Article Title: Cross-Platform Synaptic Network Analysis of Human Entorhinal Cortex Identifies TWF2 as a Modulator of Dendritic Spine Length

doi: 10.1523/JNEUROSCI.2102-22.2023

Figure Lengend Snippet: Relative protein abundance of TWF2 positively correlates with spine length. A , Module 16 eigenprotein abundance exhibits a significant positive correlation with dendritic spine length. B , Module 16 eigenprotein abundance is significantly positively correlated with thin spine length. Each point represents one case. N = 45 (17 Control, 6 CAD, 22 AD). C , Gene ontology (GO) for Module 16 indicates involvement in protein folding. Module 16 eigenprotein abundance does not differ among control, CAD, and AD synaptosomes. N = 57 (19 Control, 7 CAD, 31 AD). Error bars indicate the 25th and 75th percentiles. D , The top 6 hub proteins for Module 16 are shown. TWF2 is the top hub protein. E , Log 2 -transformed relative protein abundance of TWF2 positively correlates with spine length. F , Log 2 -transformed relative protein abundance of TWF2 was plotted against thin spine length. For E , F , points represent individual cases and the best fit line was determined via linear model. For A , B , E , F , correlations were assessed by biweight midcorrelation (bicor).

Article Snippet: Primary antibodies: PSD95 rabbit monoclonal (catalog #3450, Cell Signaling Technology, RRID: AB_2292883 ), synaptophysin rabbit monoclonal (catalog #5461, Cell Signaling Technology, RRID: AB_10698743 ), GAPDH mouse monoclonal (catalog #MAB374, MilliporeSigma, RRID: AB_2107445 ), anti-human tau rabbit polyclonal (catalog #A0024, Agilent, RRID: AB_10013724 ), TWF2 rabbit polyclonal (catalog #NBP2-47591, Novus Biologicals, RRID: AB_2895596 ).

Techniques: Quantitative Proteomics, Control, Transformation Assay

CRISPRa targeting Twf2 increases thin spine length. A , Schematic of CRISPR activation (CRISPRa) targeting Twf2. CRISPR guide RNAs (gRNAs) target the transcriptional activator, dCas9-VPR, to the target sequence to upregulate transcription of the gene of interest, Twf2. B , Schematic of the CRISPRa guide RNA used to target Twf2. The gRNA is expressed under the control of a U6 promoter. The construct co-expresses mCherry under an EF-1α promoter. C , Representative images showing colocalization of dCas9-VPR (FLAG) and CRISPR gRNAs (mCherry) in DIV14 rat primary hippocampal neurons. Scale bar = 50 µm. D , CRISPRa targeting Twf2 increases Twf2 mRNA 5-fold compared with lacZ. Unpaired t test ( t (6) = 4.320, p = 0.005). N = 4 lacZ and 4 Twf2. E , Western blotting for TWF2 on rat primary hippocampal neuron lysates demonstrates increased TWF2 levels following CRISPRa targeting Twf2. J , Quantification in F reveals that CRISPRa targeting Twf2 increases TWF2 protein 3-fold in rat primary hippocampal neurons, compared with lacZ nontargeting control. Unpaired t test ( t (4) = 14.57, p = 0.0001). N = 3 lacZ and 3 Twf2. G , Representative image showing single hippocampal pyramidal neuron transfected with Lifeact-GFP. Nuclei in blue. Scale bar = 100 µm. H , Representative images showing colocalization of lacZ or Twf2 gRNAs with Lifeact-GFP (images 1–3), as well as the deconvolved image (image 4) and 3D reconstruction (image 5). Scale bar = 5 µm. I , Overall dendritic spine length was not altered by CRISPRa targeting Twf2 in rat primary hippocampal neurons, compared with lacZ control. J , Elevating TWF2 abundance increased thin spine length in rat primary hippocampal neurons compared with CRISPRa targeting lacZ. Mann–Whitney U test ( U = 7.0, p = 0.0019). N = 8 lacZ and 10 Twf2. K , CRISPRa targeting Twf2 had no effect on mushroom spine length, in comparison to the lacZ nontargeting control. L , Dendritic spine head diameter was comparable following CRISPRa targeting of lacZ and Twf2. M , Dendritic spine density did not differ following CRISPRa targeting of Twf2 or lacZ. N , Mushroom spine density is greater than thin spine density in rat primary hippocampal neuron cultures, with no effect of CRISPR manipulation on the density of each spine subclass. Two-way ANOVA (main effect of spine subclass: F (1,36) = 101.4, p = 5.1 × 10 −12 ) with Šídák's multiple comparisons test. lacZ **** p = 5.0 × 10 −6 , Twf2 **** p = 3.2 × 10 −9 . For I–N , N = 9 lacZ and 11 Twf2. Each point represents one dendrite. Error bars represent the SD.

Journal: The Journal of Neuroscience

Article Title: Cross-Platform Synaptic Network Analysis of Human Entorhinal Cortex Identifies TWF2 as a Modulator of Dendritic Spine Length

doi: 10.1523/JNEUROSCI.2102-22.2023

Figure Lengend Snippet: CRISPRa targeting Twf2 increases thin spine length. A , Schematic of CRISPR activation (CRISPRa) targeting Twf2. CRISPR guide RNAs (gRNAs) target the transcriptional activator, dCas9-VPR, to the target sequence to upregulate transcription of the gene of interest, Twf2. B , Schematic of the CRISPRa guide RNA used to target Twf2. The gRNA is expressed under the control of a U6 promoter. The construct co-expresses mCherry under an EF-1α promoter. C , Representative images showing colocalization of dCas9-VPR (FLAG) and CRISPR gRNAs (mCherry) in DIV14 rat primary hippocampal neurons. Scale bar = 50 µm. D , CRISPRa targeting Twf2 increases Twf2 mRNA 5-fold compared with lacZ. Unpaired t test ( t (6) = 4.320, p = 0.005). N = 4 lacZ and 4 Twf2. E , Western blotting for TWF2 on rat primary hippocampal neuron lysates demonstrates increased TWF2 levels following CRISPRa targeting Twf2. J , Quantification in F reveals that CRISPRa targeting Twf2 increases TWF2 protein 3-fold in rat primary hippocampal neurons, compared with lacZ nontargeting control. Unpaired t test ( t (4) = 14.57, p = 0.0001). N = 3 lacZ and 3 Twf2. G , Representative image showing single hippocampal pyramidal neuron transfected with Lifeact-GFP. Nuclei in blue. Scale bar = 100 µm. H , Representative images showing colocalization of lacZ or Twf2 gRNAs with Lifeact-GFP (images 1–3), as well as the deconvolved image (image 4) and 3D reconstruction (image 5). Scale bar = 5 µm. I , Overall dendritic spine length was not altered by CRISPRa targeting Twf2 in rat primary hippocampal neurons, compared with lacZ control. J , Elevating TWF2 abundance increased thin spine length in rat primary hippocampal neurons compared with CRISPRa targeting lacZ. Mann–Whitney U test ( U = 7.0, p = 0.0019). N = 8 lacZ and 10 Twf2. K , CRISPRa targeting Twf2 had no effect on mushroom spine length, in comparison to the lacZ nontargeting control. L , Dendritic spine head diameter was comparable following CRISPRa targeting of lacZ and Twf2. M , Dendritic spine density did not differ following CRISPRa targeting of Twf2 or lacZ. N , Mushroom spine density is greater than thin spine density in rat primary hippocampal neuron cultures, with no effect of CRISPR manipulation on the density of each spine subclass. Two-way ANOVA (main effect of spine subclass: F (1,36) = 101.4, p = 5.1 × 10 −12 ) with Šídák's multiple comparisons test. lacZ **** p = 5.0 × 10 −6 , Twf2 **** p = 3.2 × 10 −9 . For I–N , N = 9 lacZ and 11 Twf2. Each point represents one dendrite. Error bars represent the SD.

Article Snippet: Primary antibodies: PSD95 rabbit monoclonal (catalog #3450, Cell Signaling Technology, RRID: AB_2292883 ), synaptophysin rabbit monoclonal (catalog #5461, Cell Signaling Technology, RRID: AB_10698743 ), GAPDH mouse monoclonal (catalog #MAB374, MilliporeSigma, RRID: AB_2107445 ), anti-human tau rabbit polyclonal (catalog #A0024, Agilent, RRID: AB_10013724 ), TWF2 rabbit polyclonal (catalog #NBP2-47591, Novus Biologicals, RRID: AB_2895596 ).

Techniques: CRISPR, Activation Assay, Sequencing, Control, Construct, Western Blot, Transfection, MANN-WHITNEY, Comparison